[SHUTT & CHARRON] CANADIAN EXPERIMENTS WITH NITRAGIN 57 
lation were employed. The tests were conducted in galvanized iron pots 
similar to those in general use at experimental stations; the soil, made 
for the purpose from clay, sand and swamp muck, was not sterilized, in 
order that the conditions, as far as possible, might be similar to those on 
the farm. The trials were made in duplicate or triplicate, and check or 
untreated pots were sown for the purpose of comparison. Thelucerne and 
vetch failed to make sufficient growth to warrant the recording of weights 
and the analysis of the crop. During the season of 1898 the pot experi- 
ments were continued, using the Nitraginin both methods of inoculation, 
for pease and horse beans. In addition, plot experiments were instituted, 
For this purpose an area of exceedingly poor, sandy soil, practically des- 
titute of humus and nitrogen, was selected and fertilized with fair 
amounts of phosphoric acid and potash. No form of nitrogen, however, 
was added. Thecultures used on these plots were for clover, peas and 
horse beans. In both pot and plot trials both methods of inoculation 
were employed. 
The plot experiments with clover and horse beans, using “seed in- 
oculation,” were continued in 1899, the soil employed being practically 
pure sand which had been fertilized with potash and phosphoric acid. 
Unfortunately, owing to the lateness of the season when the experiments 
were started, due to a delay in receiving the Nitragin, the plants were 
not sufficiently grown at the close of the summer to allow data of value 
being obtained. Some exeeedingly interesting and important results, 
however, were observed and recorded on the crop of inoculated and un- 
treated clover sown in 1898, which demonstrated the marked effect of 
Nitragin upon clover during the second year of its growth. 
In order that the conditions as far as possible should be alike for 
treated and untreated plants, thinning was practised both in the pot and 
plot experiments when the crop was above ground a few inches. By 
this means the same number of plants was left to mature in areas of equal 
size. As already stated, all the trials were made in duplicate or triplicate, 
the collective results as to weights of crop, nitrogen contained, etc., being 
taken. 
To ascertain what benefit might accrue from the use of Nitragin, 
either as increased growth or as additional storing-up of nitrogen in root 
or foliage, the laboratory work included the following determinations on 
the inoculated and untreated plants: Weight of crop, recorded separately 
for roots and foliage ; estimation of moisture, nitrogen, organic matter, 
and ash constituents. From these dataa comparison as to total yield and 
amount of nitrogen can be made between the crops, and also a knowledge 
gained as to such differences in composition as may exist in the root and 
foliage of the inoculated and untreated plants respectively. 
Instead of treating separately each year’s experiments and results on 
all the crops under investigation, it has been thought best to collect and 
