64 ROYAL SOCIETY OF CANADA 
and “ soil-inoculated ” crops. The amounts of nitrogen present are in 
proportion to the total yield of crop. We may, therefore, safely conclude 
that Nitragin had exerted a decidedly beneficial influence, and that the 
increase of growth and nitrogen assimilation shown in the foregoing data 
are fairly traceable to its action. 
The plants in all the pots appeared healthy, but those in the inocu- 
lated soil were decidedly larger and more robust than the others. It 
was also noticed that the root systems of the untreated plants were 
meagre and poorly supplied with nodules when compared with those of 
the treated plants, more especially with those on the plants growing in 
the inoculated soil. 
The figures of Table VII. show that the percentage of nitrogen is 
not larger in the plants from inoculated soil or seed than in those un- 
treated, we must conclude, therefore, as in the case of the clover and of 
the peas, that the additional nitrogen in the treated crop is due to the 
greater development of root or foliage, or both, induced by the Nitragin. 
In 1898 the results, both from the pot and plot trials, were somewhat 
unsatisfactory, and seemed to indicate that the vitality of the culture, 
through the action of time, light or warmth, had been impaired. In the 
pot trials, the largest crop was from the “soil inoculated” plants; the 
smallest from the “seed inoculated.” The amounts of nitrogen were 
practically the same in the untreated and “soil inoculated” plants, and 
slightly higher than in the “seed inoculated” plants. 
In the plot trials the seed was sown June 13th and the plants allowed 
to grow until October 10th. The figures obtained are as follows : 
From Untreated From Inoculated 
Seed Seed 
Weichtofbean plants, green #7" + "7." 765°4 grams 751°2 grams 
se ve vi AIT kp Kevt Les 5 515. ao De 19874 °* 198-420: 
These give further evidence that the Nitragin was, in the case of the 
beans, ineffective, due probably to one of the causes already referred to. 
Reviewing this work, it will be seen that the results are, with the ex- 
ception of the last recorded experiment, eminently~ satisfactory, furnish- 
ing evidence of a marked character as to the value of this inoculating 
agent. The experiments with clover Nitragin and pea Nitragin have in- 
variably given higher yields for treated plants, as against those grown in 
similar soil and under like conditions, but not treated with Nitragin. With 
horse beans, in 1898, we failed to obtain results that would decisively 
show a benefit from inoculation, but in the season previous a notice- 
able increase followed inoculation, pointing therefore to a deterioration 
of the particular sample of the preparation used in that year rather than 
to its general uselessness. There seems indeed no doubt from these data 
but that when fresh Nitragin is employed we are furnishing the legumes 
