Chapter 10 



Limited Heterotrophy of Some 

 Photosynthetic Dinoflagellates* 



L. Provasoli and J. J. A. McLaughlin 



STRAINS USED 



a 



^yrodiniiim californicum Bursa. A unialgal culture kindly given 

 us by Dr. B. Sweeney was purified by repeated micropipette 

 washings. She had cultured it from waters in Sorrento Slough 

 during bloom condition (10,000 cells/ml); in the sea off Scripps 

 pier only a relatively few were present. The Slough, situated 

 at the mouth of a small river near La Jolla, California, is dry 

 most of the year and divided from the sea by a sandbar; at high 

 tides the sea overflows the bar. In previous publications this 

 organism was referred to as Gyrodinium sp. and was later identi- 

 fied by A. Bursa (10, 13, and Bursa, in preparation). 



Extiviaella cassiibica, Wolosz., Peridinium chattoni Biecheler, 

 and P. halticum, Lemm. were abundant in a brackish pond at Sip- 

 pewisset. Cape Cod, Mass. Potamogeton pectinnta and Ruppia 

 maritima were also growing in the pond which had a salinity' of 

 0.63 per cent. The dinoflagellates were purified in 1955 by micro- 

 pipette washings. Gymnodinium splendens Lebour, and Gyrodin- 

 ium res})Jendens Hulburt, were purified from waters off Mamaro- 

 neck, Long Island Sound in 1954. G. resplendens was first washed 

 six times, then grown in enriched seawater containing 200 units/ 

 ml of penicillin and 1 unit/ml each of chloramphenicol, neomycin, 

 and polymixin B; after fifteen days growth in antibiotics, the 

 culture was bacteria-free (purified by Mrs. Susan Egloff). G. 

 splendens was purified by micropipette washings. 



Gyrodinium uncatemtm Hull^urt, kindh' given us by Dr. 



* This work was supported in part by contract NR 104-202 with the Office of 

 Naval Research and b>' grant B-1I98 from the National Institutes of Health. 



105 



