TABLE 1 



Composition of Various Media Used for the Isolation, Maintenance, 



AND Testing of Aerobic, Heterotrophic Bacteria from the Sea. 



The pH is Adjusted to 7.2 



Ingredients 



Artificial 

 Sea Water 



A 



Isolation 



and 



Maintenance 



B*** 



Vitamin 

 Tests 



C 



Minus 

 Amino 

 Acids 



Distilled water 



NaCl 



Na2S04 



NaHCOa 



KCl 



KBr 



H3BO. 



MgCl.-6H,0 



SrCl2-6H,0 



CaCl2-2H.O 



Trypticase 



Soytone 



Yeast extract 



Marine mud extract* 



Natural sea water 



Fe(NH4)2(S04)2-6H20 



Difco agar** 



Artificial sea water 



Casamino acids (-vitamins) 



Dextrose 



Na succinate 



Cystine 



Tryptophane 



K2HPO4 



Adenine 



Cystosine 



Guanine 



Thymine 



Uracil 



Xanthine 



Biotin 



Cobalamine 



Nicotinic acid 



Pantothenate 



Riboflavin 



Thiamine 



NH4NO3 



To 1 1 



23.476 gm 

 3.917 gm 

 0.192 gm 

 0.664 gm 

 0.096 gm 

 0.026 gm 



10.610 gm 

 0.040 gm 

 1.469 gm 



2gm 



2gm 



2gm 



100 ml 



900 ml 



15 gm 



1 figm 



7 mg 



To 1 1 



5 gm 

 2gm 

 2gm 

 100 mg 

 100 mg 

 50 mg 

 5 mg 

 5 mg 

 5 mg 

 5 mg 

 5 mg 

 5 mg 

 1 figm 

 1 /"gm 

 500 yugm 

 800 /jigm 

 500 /igm 

 800 figm 



7 mg 



To 1 1 



2gm 

 2gm 



50 mg 

 5 mg 

 5mg 

 5mg 

 5 mg 

 5mg 

 5 mg 

 1 /igm 

 1 M&m 

 500 figm 

 800 figm 

 500 ^gm 

 800 figm 

 0.5 gm 



* Mud extract is prepared by autoclaving 1 kilogram of wet marine mud 

 together with 1 liter of sea water for 20 minutes and filtering. Preserve under 

 toluene and store in a refrigerator. 

 ** Difco purified agar, 15.0 gm per liter, was used in making media for vitamin 

 tests in plates. 

 *** Vitamins are omitted from this medium in appropriate fashion, depending 

 upon the experimental design. 



