372 



Marine Microbiology 



HOURS 



Fig. 2. Growth of clone T16 in tissue cultures of marine fish fin cells versus 

 growth in Melnick's mammalian tissue culture medium. 



Melnick's mammalian medium seemed to be hypotonic to T16, 

 the cells looked swollen and rounded, while in seawater cultures 

 they have a clearly outlined sigmoid elongated shape. 



Virus Experiments 



When we combine in one cultural system three biological 

 systems— ciliate, food cell and virus— we have to expect that they 

 act upon each other and react with each other. A careful study 

 has to be made as to balancing out all experimental factors such 

 as temperature, duration of incubation, salinities of the media, 

 physiological condition of the ciliate and the food cell, etc. 

 Several supporting experiments preceded the actual infection 

 experiments. 



It was found that poliovirus strain Mahoney is temperature 

 stabile within certain limits. Inactivation took place in samples 

 suspended in Eagle's maintenance medium over a period of 11 



