Studies on a Marine Parasitic Ciliate 



373 



TABLE 1 



Effect of Temperature on Final Cell Yields of Strain T5 and T16 (50ml 

 Cultures of Melnick's Medium, Speed Level 1,000 cil/ml. Seven Days 



Culture Period) 



TABLE 2 



Averages of Final Yields of Twelve Successive Passages of Strains T5 



and T16 in Media of Different Salinities (50 ml Cultures, Seed Level 



1,000 cil/ml. Seven Days Culture Period) 



T5 

 20 C 29 C 



T16 

 20 C 29 C 



Melnick's Mammalian 



medium (320 mOsM) 161,950cil/ml 122,527cil/ml 66,425cil/ml 44,783cil/ml 



Melnick's Seawater 



medium (983 mOsM) 46,958 



45,333 



31,463 29,587 



days in the following order: at 23 C— 50 per cent loss, at 30 

 C— 90 per cent loss and at 37 C— 99 per cent loss. Tliis informa- 

 tion helped to find the proper dosages to be used and to deter- 

 mine the duration and incubation temperatures of future experi- 

 ments. 



The results of incubation of poliovirus suspended in several 

 media of various salinities are tabulated in Table 3. There was 

 no significant difference in effect of the various media on \'irus 



