402 



Marine Microbiology 



d 



.02 - 



.0 I 



.00 



Q 



o 



. .02 - 



450 500 550 



A (nn|jL) 



600 



Fig. 3. {Above) Steady state-oxidized difference spectra of intact Nitro- 

 bacter cells and cell-free fractions P-144,000 and S-144,000 upon addition 

 of KNO.,. {Below) Steady state-oxidized difference spectra of cyanide dial- 

 yzed and control dialyzed fractions P-144,000 upon addition of KNOo and 

 FeSO^. The (+NOo") and -f NO^- + Fe++) curves are for cyanide-dia- 

 lyzed fraction P-144,000. 



Phosphate esterification has been shown to be specifically coupled 

 to the enzymatic oxidation of nitrite by the cytochrome- 

 containing electron transport particle which catalyzes the forma- 

 tion of high energy phosphate bonds in the form of ATP (6). 

 Phosphorylation does not occur in the absence of the oxidizable 



