404 Marine Microbiology 



TABLE 1 



Factors Affecting Oxidative Phosphorylation by Particulate 

 Nitrite Oxidase* From N.agilis 



* Resuspended pellet collected from 27,000 x g-supernatant by centrifugation at 

 95,000 X g. The complete reaction mixture contained 10 micromoles phosphate, 

 25 micromoles nitrite, 0.2 micromoles ADP (PABST Co.), 15 micromoles 

 MgCU; 0.5 mgs hexokinase (type III Sigma Co.,), 50 micromoles glucose, 0.5 

 ml nitrite oxidase corresponding to 0.6-1.5 mg protein, P^- (phosphatope oral, 

 without preservative, without carrier - E. R. Squibb and Sons, New York) 

 approximately 3 x 10^ cpm per Warburg flask. 



The final volume was made up to 3.2 ml with O.IM Tris pH 7.5. The 

 central well contained filter paper and 0.2 ml of 10 per cent NaOH. After a 

 ten minute preincubation period at 30 C, the reaction was started by tipping 

 in the enzyme from the side arm and Os-uptake determined at ten minute 

 intervals for one hour. 



t These values represent the consumption of O2 by the total volume of reaction 

 mixture (3.2 ml) contained in the Warburg flask. 



X These values represent the actual counts per min measured in the organic 

 phosphate fraction in an aliquot equivalent to 1/48 of the volume of the 

 total reaction mixture (3.2 ml) contained in the Warburg fla.sk. 



** P/O ratios are corrected for by subtracting the endogenous O2 and P^^ 

 uptakes obtained in the minus nitrite reaction mixture. 



