444 Marine Microbiology 



active substance commonly present in animal, plant and microbial 

 tissues and has not yet been examined with this respect in the 

 previous works, for its ability to stimulate growth of a salt water 

 strain of D. desulfuricans (strain Maizuru 1). 



TABLE 1 



Effect of ATP on the Growth oj Desulfovibrio desulfuricans (Maizuru 



1) IN A Defined Medium Supplemented with Peptone or Casamino Acids 



OR Eighteen Amino Acids as Nitrogen Sources 



Supplement* Growth, O. D. at 610 mfi 



Peptone 0.39 



Peptone + ATP 0.40 



Casamino acids 0.06 



Casamino acids + ATP 0.34 



18 amino acids 0.05 



18 amino acids + ATP 0.42 



None (basal medium)* 0.00 



* Basal medium was composed of minerals, lactate and asorbic acid only. 



The data illustrated in Table 1 are the results of experiments 

 carried out to demonstrate the effect of ATP on the growth of 

 this microorganism in media based on metallic salts, sulfate, 

 lactate and L-ascorbic acid, and supplemented as nitrogen 

 sources with peptone or Casamino acids or a mixture of eighteen 

 amino acids which was composed of L-arginine, L-histidine, L- 

 lysine, L-tryptophane, DL-tyrosine, L-phenylalanine, L-cystine, 

 DL-methionine, DL-serine, DL-threonine, L-leucine, L-isoleu- 

 cine, L-valine, L-glutamic acid, L-aspartic acid, glycine, DL- 

 alanine, and L-proline. 



When supplemented with ATP, the partially defined medium 

 containing Casamino acids as nitrogen sources enabled good 

 multiplication of the microorganism tested, whereas very little 

 growth occurred in such a medium without added ATP. The 

 growth obtained in the Casamino acids medium with ATP was 

 similar to that in the peptone medium. The replacement of 

 Casamino acids by a mixture of eighteen amino acids in the 

 medium supplemented with ATP also supported the normal 

 growth which was approximately equal to that in the peptone 



