446 



Marine Microbiology 



Adenosine, inosine, adenine, hypoxanthine and guanine, how- 

 ever, failed to support the normal growth. 



These facts suggest that the ability of this microorganism to 

 synthesize some nucleotides was limiting for its own growth, 

 probably at tlie initial stage. 



TABLE 3 



Effect of Amino Acids on the Growth of D. Desulfuricans (Maizuru 1) 

 IN Chemically Defined Media 



Amino Acid Omitted*** 



Growth, O. D. at 610 m/i 



DL-Alanine 



L-Arginine 



L-Aspartic acid 



L-Cystine 



L-Glutamic acid 



Glycine 



L-Histidine 



L-Isoleucine 



L-Leucine 



L-Lysine 



DL-Methionine 



L-Phenylalanine 



L-Proline 



DL-Serine 



DL-Threonine 



L-Tryptophane 



DL-Tyrosine 



L-Valine 



None 



0.01 

 0.02 

 0.01 

 0.15 

 0.03 

 0.28 

 0.31 

 0.01 

 0.01 

 0.01 

 0.33 

 0.01 

 0.13 

 0.01 

 0.01 

 0.29 

 0.31 

 0.01 

 0.34 



*** Each amino acid was omitted from the complete medium based on minerals, 

 lactate, ascorbic acid, ATP and eighteen amino acids. 



RESPONSE OF DESULFOVIBRIO DESULFURICANS 

 (MAIZURU 1) TO AMINO ACIDS 



To elucidate the response of D. desulfuricans (Maizuru 1) 

 to amino acids in detail, we examined subsequently the respec- 

 tive eflFects of the above described eighteen amino acids on the 

 growth of this microorganism, by observing its growth in chemi- 

 cally defined media based on minerals, lactate, ascorbic acid, 

 ATP and seventeen amino acids (out of the complete eighteen 

 amino acids each one to be tested was omitted respectively). 



The results of this experiment, which were illustrated in 



