Pathways of Lower Alkaline Oxidation 469 



TABLE 3 

 Dehydrogenase Activities of Extracts From Heptane Grown Cells 



Substrate 



Hydrogen Acceptor 



Activity 



n-heptane 

 n-heptane 

 n-heptane 

 1 7J-heptanol 

 1 7i-heptanol 

 n-heptanal 



Pyocyanin 



DPN 



TPN 



Pyocyanin 



DPN 



DPN 



0.58-0.70 

 0.40-0.55 



0.075 



0.95 



0.60 



0.80 



* Measured at 23°C and expressed in /imolcs of the acceptor reduced per hour, 

 per mg. of nitrogen. For DPN and TPN reduction, systems containing 0.03 M 

 mercaptoethanol. 



0.2 



.1- 



/ 



/ 



•^ 





y 



(D 



(5) 



10 



20 



30 



40 

 minutes 



Fig. 2. Reduction of DPN by cell free extracts. Quantities of reduced DPN 

 determined at 340 m^u, Curve ( 1 ) : complete system containing bacterial 

 extract (0.8 mg. protein N), DPN 0.2 /^mole, n-heptane 0.1 ml., mercap- 

 toethanol 0.03 M. Curve (2): mercapto-ethanol omitted; curve (3): n- 



heptane omitted. 



