Occurrence and Significance of Chilinoclastic Bacteria 595 



cated in pelagic areas, which are less affected by such factors as 

 terrestiial runoff, upwelling, relatively rapid currents and counter- 

 currents, etc., tliis work was done as much as practicable aboard 

 ship in pelagic areas. 



Preliminary to work at sea, efforts were made to evaluate 

 media and culturing conditions of pelagic chitinoclastic micro- 

 organisms. 



EXPERIMENTAL 



Water samples were taken from shipboard casts, using sterile 

 bacteriological J-Z water samplers, ( 28 ) . The water samples were 

 transferred asepticallv from the J-Z bulbs to sterile glass bottles, 

 and samples withdrawn from these for laboratory work aboard 

 ship. 



Zooplankton was taken in standard net tows ( 00 bolting silk, 

 one meter nets, 200 meters wire out) on Cruises 3 and 5 (Fig. 1), 

 and from an Isaacs-Kidd six-foot midwater trawl on Cruise 4. 



Figure 1 

 lX)CATIONS OF CRUISE ST/iTlONS 

 Figures In parentheses are *a(er depths 



Fig. 1 . Locations of cruise stations. 



