Host and Habitat Relationships 613 



25 C (RT) and after 25 days at C were picked off the agar into 

 tubes of seawater broth. A random selection of colonies was 

 made. The total number in the samples was governed by the 

 maximum number which could be handled at one time. The 



TABLE 1 



Coded Characteristics Used in the Study of Marine Commensal Bacteria 



short (0.2 fi - 0.6 (i) 

 medium (0.6 fi - 1.2 ju) 

 slender (0.2 n - 0.6/i) 

 stout (0.6 fi - 1.0 n) 

 rounded end 



Morphology 



rods 



curved rods 



ovals (spheres) 



filaments 



singles 



pairs 



chains 



Culture 



small colony (< 2mm) heavy turbidity 



med. colony (2-5 mm) moderate turbidity 



entire edge slight turbidity 



convex even turbidity 



white granular sediment 



off-white ring 



gray pellicle 



green pigment (diffusible) fluorescent 



opaque 



translucent 



Biochemistry 



agar digested 



gelatin liquefied 



litmus milk peptonized 



litmus milk surface peptonized 



litmus milk acid 



litmus milk alkaline 



litmus milk reduced 



litmus milk acid— > alkaline 



litmus milk alkaline—^ acid 



motile 



polar flagellation 

 Gram negative 

 Gram variable 



Physiology 

 growth at C 

 growth at 25 C (RT) 

 growth at 37 C 

 seawater required 

 catalase positive 

 penicillinase present 

 0/129 insensitive 



glucose positive (Hugh and Leifson, 1953) 



glucose acid 



glucose gas 



maltose acid 



maltose gas 



sucrose acid 



sucrose gas 



lactose acid 



lactose gas 



ammonia produced in peptone water galactose acid 



Voges-Proskauer positive 

 methyl red positive 

 indole positive 

 growth in Koser's citrate 

 urease production on 



Christiansen's urea 



agar slants 

 cytochrome oxidase positive 



(Gaby) 

 oxidase positive (Kovacs) 



galactose gas 

 mannitol acid 

 mannitol gas 

 starch hydrolyzed 

 gluconate oxidized 

 nitrates reduced to nitrites 

 nitrites reduced 



hydrogen sulfide produced from cysteine 

 trimethylamine oxide reduced to 

 trimethylamine 



