614 Marine Microbiology 



broths containing the selected colonies were incubated at 25 C 

 (RT) or C, depending upon the initial temperature of isolation. 



Each isolate was streaked three times to check purity before 

 the testing program was carried out. The pure cultures were 

 studied for moi-phological, physiological and biochemical charac- 

 teristics. 



A full description of the methods and media used in testing 

 the bacteria was given in a previous paper (3) and the tests are 

 summarized in Table 1. Altogether 48 tests were carried out on 

 each organism, and in most cases the tests were repeated at the 

 end of six months. 



Thus, there was obtained for analysis, a total of approxi- 

 mately 200 characteristics for each of 743 pure cultures taken 

 from nineteen species of fish, representing twenty genera from 



^ 30 



■ Vertebrates 



LJ invertebroles 



Totol Number of Cultures' 736 



1 



il 



ll i1 



n 



Ml 



Fig. 1. Generic distribution of bacteria found on marine animals (expressed 



as per cent). 



