X. i:i>'Ki:('i's OF i)i;fi(1i:.\cy (i()3 



or support ot normal ^ii'ow tli."' This may he aclii('\('(l hy the addition of 2 

 III .") ■) ot' liiot in ])('i- da\' or li\' I ceding 7 to 10 y of hiot in per 100 <^-. ot diet .-'* 



('. DOdS 



Puppi(vs t'cd a synthetic diet deficient in the \itamin H complex hut 

 supplemented with all known members of tills complex with the exception 

 of hiotin and folic acid developed a progressive paralysis after 7 to 48 weeks. 

 This neurological condition resjjonded, at least temporarily, to injections of 

 hiotin. •■'•^ •■'" Later it was shown that the symptoms were due primarily to a 

 l)otassium deficiency, and a single ade(iuate dose of potassium was followed 

 hy comi)lete remission lasting for (i to 10 weeks. Here an apparently latent 

 potassium d(>ficiency was accentuated by simultaneous deficiency of bio- 



1). PKJS 



Egg whit(> injury may also be pi'oduced in pigs by feeding the usual 

 experimental diet containing ai)out 30% commercial dry egg.^" Biotin defi- 

 ciency in a mild form may de\'elop^' in young piglets on a l)iotin-fi-ee ration 

 without the addition of a\idin (egg white). The syndrome in pigs is charac- 

 terized by alopecia, sel)()rrheic skin changes, spasticity of the hind legs, and 

 cracks in the feet. The same condition, in less |)ronounced form perhaps, 

 may also be produced by administration of phthalylsulfathia/.ole (and not 

 of sulfaguanidine) in a semisynthetic diet.^'- In both instances biotin j)ro\'ed 

 to l)e the specific factor of prevention or treatment.^"" '- 



E. COWS 



It has been ( laimed^-' that the dairy calf rc([uires an exogenous supply 

 of biotin. In the absence of l)iotin, paralysis of the hind legs was observed. 

 The paralysis was cured by the administration of biotin. 



F. FISH 



In young trout, biotin deticienc}' manifests itself by progressive anemia^^ 

 and the so-called "blue slime" disease.^^ In young brown trouts fed a ra- 



3«S. G. Smith, Science 100. 389 (1944 1. 



3' S. G. Smith and T. E. Lester. .4»;. J. Fhysiol. 144, 175 (1945). 



3* W. R. Ruegamer, C. A. P:ivehjem, and E. R. Hart, Proc. Soc. Exptl. Biol. Med. 61, 



234 (1946). 

 39 S. G. Smith, f'mr. Snr. Kxpll. Hiol. Mr, I. 63. ;i39 (194(5). 



" T. .J. Cunh.i. I). (". I.iii.iioy, ami M. K. KnsminKor. ./. Animal Sci. 5, 219 (1946). 

 " W. P. Lohrer, Jr., .\. C". Wiese, and P. R. Moore, ./. Xutrition 47, 203 (1952). 

 - D. C. Lindley and T. J. Cunha, J. Xutrition 32, 47 (1946). 

 ■3 .\. C. Wiese. B. C. Johnson, and W. H. Xevens, Proc. Soc. Exptl. Biol. Med. 62, 



521 (1946). 

 " M. .\. McLaren. K. H. Keller. I). J. ()4)oimell, and ('. A. Klvehjem, Arch. Bio- 



chiiN. 15, 169 (1947 . 



