X. EFFECTS OF DKFK'IKXCV Oil 



nuicli a> ihis vi]'vf\ was cxci-tcd hy uiilicatcd as well as hy healed (axidiii- 

 free) eg^ while, it must l)e independent ot' any ichition helweeii hiotin and 

 in'idin. 



I'urthennore, in mice with sixmtaneoiis inaniinar>" eai'cinoina, a diet rich 

 in e<i:<i; white and a\i(hn was without an>' heiielicial effect. Simihir iiefi;ative 

 results wcic obtained witli a ration containing liij^h levels of egg white in 

 mice with f'lexner-Johling sarcoma or mouse sarcoma 180.^' In other ex- 

 periments'-' mice were fed an a\idin-coiitainiii»>; diet until they developed a 

 severe biotin deliciency with a simultaneous reduction of the biotin levels 

 in the tissues 80 to 90% below normal. Fi'agments of sarcoma 37 or 180 

 were inti'oduced subcutaneously into these biot in-deficient mice as well as 

 into normal control mice. All the tumors in l)oth groups of animals grew 

 well and were large and healthy, indicating that severe biotin deficiency 

 was without any effect on the propagation of the tumor tissue. 



Attemi)ts were also made to influence cancerous growth in man by a 

 diet containing excessive amounts of egg white or a\idin. The results were 

 disappoint ing]\' negative.^"'- ^* 



L. HIOTIX AXl) RESISTANCE TO DISEASE 



Trager'*'' • ''■'' has shown that biotin deficiency causes both a piolongation 

 and an increased .severity of infection with Plas:mo(h'u)n lophurac in chick- 

 ens and with the same a\'ian malaria Plasmodium or with P. rathemrrinm 

 in ducks. A diet deficient in |)antotlienic acid had no effect on tlu> malaria 

 infections in chickens. Caldwell and (Jyorgy have r'^poi'ttni a significant 

 j)rol()ngation of infection with TrijpdNosoma Icwisi in the albino rat as a 

 result of biotin deficiency. ^^ Seeler ct a/.-^ confiimed Trager's findings on 

 liiotin deficiency and a\-ian malaria. On the othei' hand, Seeler and Ott^*^ 

 observed that ribofla\'in deficiency decreased the se\'erity of infection with 

 P. lophurac in the chick although the infection was not .shortened. Hiotin 

 deficiency also decreased the resistance of mice to Salmonella lyphi'tnurium 

 infectioiT-' but had no effect on the susceptil)ility of Swiss mice to experi- 

 mental lioliomyelitis.''" 



s' C. J. Kcuslor, C. Wad.sworth. K. Suguira. C. P. Hfioads. K. Dittmcr. and V. du 



Vigiieaud, Cancer Research 3, 823 (1943). 

 « C. P. Rhoads and J. C. Abels, ./. Am. Med. Assoc. 121, 1261 (1943). 

 «3 I. I. Kaplan, .4m. ./. .l/c^/. Sci. 207, 733 (1944). 

 «^ W. Tracer, ./. Expll. }[ed. 77, 557 (1943). 

 ^' W. Tracer, ./. Exptl. Med. 85, 663 (1947). 



*6 F. E. Caldwell and P. riyorgy. Proc. Soc. Expll. Biul. Mid. 53, IKi (1943). 

 " A. O. Seolcr. W. II. Ott, .uid .M. K. Gundel, Pruc. Soc. Expll. Biid. Med. 55, 1(17 



(1944). 

 **" .\. O. Seeler and \V. liOtt. ./. Infectin„i< Diseases 75, 17.") (1944). 

 "^ I. J. Klujilfi-, K. ( lutiiiciilicini. ,ind II. Hcri'idicisci-. ./. fnfrrlions />/.s7Y/.s-r.v 78, 60 



(1946). 



