VIII. EFFECTS OF DEFICIENCY 



35 



decrease in PABA synthesis by Sacchai-omijces cerevisiae from 42 7 per liter 

 to 0.83 7 per liter. The specific vitamin concerned with this effect on 

 PABA synthesis was not established. 



Agarwala and Peterson-^ found a depression of PABA synthesis with 

 four j^easts when the vitamins thiamine, pyridoxine, biotin, pantothenic 

 acid, nicotinic acid, and inositol were added to a synthetic medium. Thia- 

 mine appears to be the factor most concerned in influencing PABA synthe- 

 sis, since reductions from 124 7 per gram to 41 7 per gram and from 1 1.8 7 

 per gram to 2.7 7 per gram were observed with Candida krusoides and Torula 

 utilis, respectively. 



TABLE VI 

 Microorganisms with a Growth Factor Requirement for PABA 



Microorganism 



Approximate 

 PABA require- 

 ment, 

 7/ml. 



References 



Acetobacter suboxydans 



Chlamydomonas Moewusii mutant 



Clostridium acetobidylicum 



Clostridium butylicum 



Clostridium, felsineum 



Clostridium sporogcnes 



Clostridium thermosaccharolyticum 



Corynebacteri iim diphtheriae gravis (Dundee) 



Escherichia coli mutants 



Lactobacillus arabinosus 



Leuconostoc mesenteroides P-60 



Neurospora crassa mutant 



Ophiostoma multiannulatxmi mutant 



Plasmodium knowlesi 



Propionibacterium sps. 



Rhizobium trifolii 



Rhodopseudomonas palustris 



Rhodotorula aurantiaca 



Saccharomyces cerevisiae 



" Not specified. 



" S. C. Agarwala and W. H. Peterson, Arch. Biochem. 27, 327 (1950). 



23 L. A. Undcrkofler, A. C. Bantz, and W. H. Peterson, /. Bactcriol. 45, 183 (1943). 



-* J. (). Lami)on, L. A. Underkotter, and W. 11. Peterson, ./. Biol. Chcm. 146, 277 



(1942). 

 25 J. H. Marshall and .1. R. Postage, Proc. 1st. Int. Conrjr. Biochem. \). .338 (1949). 

 2« R. A. Lewin, Nature 166, 196 (1950). 



" J. O. Lampen and W. H. Peterson, Arch. Biochem. 2, 443 (1943). 

 2* R. D. Housewright and S. A. Koser, ./. Infectious Diseases 75, 113 fl944). 

 29 S. D. Rubho and J. M. Gillespie, Nature 146, 838 (1940). 



