IX. EFFECTS OF DEFICIENCY 



ISl 



and ;> million arc coininon, and counts of 1 million ov slijililly less have, been 

 obscixcd. The anemia is sli<2;hlly macrocytic, the \()lumc index and mean 

 cell diameter beiujj; sli«i;htly increased. 



'I'he addition of PGA, or concentrates of tliis vitamin, effects ti reticu- 

 locyte ci'isiswhich may ])e as high as 40 %, a ch'amati(^ increase in \vhit(^ cells, 



220 240 260 



Days on experiment 



Fig. 12. Typical responses of the cytopenic monkey to natural pterojltriglutamic 

 acid (L. casei factor). In each of the two experiments on this monkey the total dose of 

 vitamin was appro.ximately .3 mg., given intramuscularly. Courtesy J. Biol. Chem. 

 (Daye/a/."). 



and a slo\ver return of red cell and hemoglobin values. ^^ A typical response 

 of a deficient monkey to administration of pteroyltriglutamic acid (fer- 

 mentation L. casei factor) is shown in Fig. 12. 



A megaloblastic bone marrow is found in monkeys with nutritional cyto- 

 penia. Wilson^^ reported that there is a relative hypoplasia in the myeloid 

 elements in monkeys in the terminal stages of peripheral leucopenia, 



" P. L. Day, V. Mims, and J. R. Totter, J. Biol. Chem. 161, 45 (1945). 

 5« H. E. Wilson, S. Saslaw, J. L. Schwab, O. C. Woolport. and C. A. Donn, ./. Am. 

 Med. Assoc. 121, 1411 (1943). 



