PLATF. I. 



Alt the figures in (his plate are drawn from preparaii( 

 without aceiic acid), and stained by Mann's methyUjhie-ei 

 technique is indicated. Magnification 2,500 diameters. 



; 6xed with sublimate-alcohol (with or 

 n method (modified), unless a diflTerent 



Kit-. 

 Fiiis. 



Figs. I — 6. Entamoeba histolytica. 



1. E. histolytica, active amoeba scraped from an intestinal ulcer (cat), showing typical 

 structure of nucleus, and partly digested remains of three ingested red corpuscles. (Fixed 

 in Houin's fluid.) 



2. Precystic amoeba, from human stool (subacute amoebic dysentery). 



5, 4, 5. Uninucleate, hinucleate, and quadrinucleate cysts respectively : from same case as 

 preceding. Strain forming cysts (living) with mean diameter of ca. I3'5|U. Note nuclei, 

 chromatuid bodies (red), and clear spaces in 3 and 4 representing glycogen vacuoles. 



Fived with CarnoyVs fluid, stained 



Figs. 7, 8, 9. /indolifi. 



Fig. 13. Precystic amoeba, from same case. Note nuclear structure, absence of ingested food, 

 etc. (and compare with fig. 2). 



Fig. 14. Mature 8-nucleale cyst, from same case. Note nuclear structure (compare with figs. 12 

 and 13, and figs. 1—5), and small chromatoid body (red). 



Fig. 15. Binucleate cyst, containing large glycogen mass (red). Fixed in Carnoy's fluid, stained 

 with Best's carmine. (Cf. figs. 6 and i).) 





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