PLATE 1\/. 



All figures drawn— unless otherwise staled— frofT 

 stained as indicated. Magnification 2,500 dia 



Figs. 55—69. Entamoeba coli. 

 Fig. 55. Active E. coH from human stool, showing "clefts" 

 iron-haematoxylin.) 



Figs. 56—69. Cysts of E. coli at vario 



ublimate-alcohol anJ 



etc. (Weigeri's 



Fig. 56. Newly-formed t 

 {Stained haemalun 



Fig. 57. Later cyst, with very largi 

 bodies and glycogen "vacuole.' 



ucleate cyst, showing minute chn 



in development. 



latoid bodies, glycogen space, etc. 



Fig. 58. First nuclear spindle 



(Man. 

 (Heidenhain'i 



; preparing for first 

 I's stain, modified.) 



i-haematoxy1in and 1 



Note chromatdid 



Figs. 59, 60. Binucleate cysts. Note glycogen *' vacuoles," chromatoid bodies, etc. (Compare 

 with tig. 15, PI. I.) Fig. 59 from cyst stained by Mann's method (modified): Fig. 60, 

 haemalum. 



Fig. 61. Quadrinucleate cyst. Note that all nuclei are in early stages of division — as they 

 usually are in 4-nucleate cysts of this species. Fixed Bouin's fluid, stained alcoholic ferric- 

 chloiide iron-haematein. 



e). Note structure of resting nuclei. (lieidenhain's 



Fig. 62. Typical 8-nucleate cyst (m 

 iron-haematoxylin.) 



Figs. 63—66. E. coli cysts, at various slages of development, containing chromatoid bodies of 

 different forms. Fig. 65 shows a mature cyst with filameniar chromatoids — probably a cyst 

 of the type attributed by Prowazek to " Entamoeba 2viiliamsi." (All these cysts stained 

 with haemalum, progressively.) 



Fig. 67. Abnormal i6-nucleate cyst of E. coli. (Stained with haemalu 



Fig. 68. Very small 8-nucIeate cyst of E. coli. (Haemalum and eosin.] 



Fig. 69. Very small 4-nucleate cyst of E. coli, containing 4 resting i 

 Compare with fig. 71, etc. (Haemalum and eosin.) 



iclei and chromatuid bodic 



Figs. 70—76. Entamoeba hitolytic 



Fig. 70. Cyst of E. histolytic 

 (Fixed alcoholic picro-acc 



(uninucleale), containing very abundant chromatoid bodies, 

 ic-acid, stained paracarmine.) 



Fig. 71. Mature 4-nucleate cyst, without chromatoid bodies. (Haemalum.) Compare with 

 fig. 69. 



Figs. 72, 73, 74. Three very small cysts of E. A/j/o/y/iVa— uninucleate, binucleate, and quadri- 

 nucleate respectively— belonging to a strain producing cysts (living) with an average 

 diameter of 6-6 >(. (Haemalum.) 



Figs. 75. 76. Two very large cysts of E. /;/j/<?/y//Va— respectively uninucleate and quadrinucleate 

 —belonging to a strain forming (living) cysts with an average diameter of 15 /*. (Haemalum ) 

 Compare with preceding figs. 72-74, and with figs, of E. coli on this plate. 



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