Chapter VII 



Intracellular Multiplication of Bacterial Viruses 



GuNTHER S. Stent 

 Virus Laboratory, University of California, Berkeley, California 



La bacteriologie rCa jusquHci envisage que le ^probleme des deux corps,'' bacterie et 

 milieu, que ce milieu soil Vorganisme parasite ou le liquide au sein duquel se fait la 

 culture, et ce probleme etait deja bien complexe; il Vetait pourtant beaucoup rrwins que 

 le ''probleme des trois corps' que nous allons aborder, ou nous aurons a envisager les 

 interactions entre le milieu, milieu de culture ou organisme parasite, la bacterie 

 parasitant ce milieu el Vultramicrobe ba^cteriophage parasite dans la bactirie. d'Herelle 

 (1921). 



I. Introduction 238 



II. Kinetics of Phage Reproduction 239 



A. One-Step Growth 239 



B. Single Burst 242 



C. Lysis and Lysis Inhibition 243 



D. The EcUpse 246 



III. Synthesis of the Bacteriophage Progeny Substance 249 



A. Incomplete Bacteriophages 249 



1. Sedimentation, Adsorption, and Precipitation 249 



2. Serum-Blocldng Power 251 



3. Morphology 252 



4. Phage-Specific Nucleic Acid 253 



B. The Precursor Pool 254 



1. Origin of Progeny Nucleic Acid 254 



2. Origin of Progeny Protein 257 



C. Maturation 258 



1. Phenotypic Mixing 258 



2. Proflavine 259 



IV. Synthesis of Nonprecursor Materials 260 



A. Ribonucleic Acid 260 



B. "Early" Protein 261 



V. Fate of the Infecting Phage Particle 263 



A. Functional Differentiation and Injection 263 



B. Transfer of the Parental Nucleic Acid 265 



1 . Extent and Specificity of Transfer 265 



2. Distribution of Transferred Material 268 



C. Superinfection Breakdown 269 



VI. Comparative Bacterial Virology 270 



VII. The Vegetative Phage 274 



References 276 



237 



