BIOCHEMISTRY OF INSECT VIRUSES 521 



B. mori virus obtained by Berk ef eld filtration is adsorbed on kaolin 

 (negatively charged) and on aluminium hydroxide (positively charged) at a 

 pH range between 5.0 and 6.0. It can be eluted with 0.2 M phosphate buffer 

 of a pH of 9.0-9.5, but loses a considerable degree of its infectivity (Watanabe, 

 1941a). It was found that the virus migrates in the electrical field towards 

 the cathode at a pH of 4.5-5.7 (positively charged) and to the anode between 

 pH 6.9-10.0 (negatively charged) but does not move between pH 5.8-6.3 

 (isoelectric) (Watanabe, 1941b). In precipitation experiments with poly- 

 hedron solutions, it was found that the isoelectric point of the virus is at pH 

 6.0-6.2 (Aizawa, 1952). 



The above results indicate that some classic insect viruses are fairly well 

 investigated, but that we know nothing or very little about the chemical 

 composition of the other groups of insect viruses, for example, the cr-virus 

 of Drosophila and the so-called insect-plant viruses. 



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