VIII. EFFECTS OF DEFICIENCY 



295 



depleted adult rats by restricting food intake until the animals were half 

 their starting weight. When the animals were then fed fat-free diet ad 

 libitum, classical symptoms of fat deficiency appeared. These symptoms 

 could be prevented or cured by ethyl linoleate. The symptoms disappeared 



Fig. 8. (h) Dog maintained on low fat diet for 7 nunths, then given diet contain- 

 ing 5% of total calories as fat for 3 months. At 7 months this animal showed sim- 

 ilar symptoms to those illustrated in (a). (From Hansen and Wiese.^') 



spontaneously after prolonged periods of ad libitum feeding of fat-free diet, 

 indicating the possibility of synthesis of some essential fatty acid. 



2. Dog 



The fat-deficiency syndrome has been observed in a variety of animals. 

 Hansen and Wiese""" have made extensive studies in fat deficiency of dogs. 



•> A. F. Hansen and H. F. Wiese, Texas Rcpts. Bi,>l. Med. 9, 491 (l<)r)l). 

 '= H. F. Wiese and A. E. Hansen, Texas Repts. Biol. Mrd. 9, 516 (1951). 

 '3 H. F. Wiese and A. E. Hansen, Texas Repts. Biul. Med. 9, 545 (1951). 

 '4 A. E. Hansen, S. G. Holmes, and H. F. Wiese, Texas Repts. Biol. Med. 9, 555 (1951). 



