III. BIOCHEMICAL SYSTEM 623 



TABLE VIIPs 



Lipid Synthesis with CoA-Poor and CoA-Rich Resting Cells 



OF Saccharomyces cerevistae'^ 



° Identical pantothenic acid-deficient yeast samples were incubated with and without pantothenic acid 

 for 2 to 3 hours, washed, and assayed for CoA. Subsequently, the yeast samples were incubated for 16 hours 

 in an acetate-phosphate medium and analyzed for total lipid and steroid. 



acceptor territory and derive acetyl from acetyl-CoA. In confirmation a 

 still rather general connection between CoA and fat and steroid synthesis 

 has evolved from studies in this laboratory with Klein. *^ By a comparison 

 of CoA-poor and CoA-rich yeast samples (Table VIII), he showed that 

 that fat and ergosterol synthesis runs parallel with CoA concentrations. 

 A similar parallel between fat and cholesterol synthesis and CoA levels 

 was obtained in experiments with pantothenic acid-deficient rats.^* The 

 incorporation of isotopic acetate into fat and cholesterol in liver slices from 

 normal and pantothenic acid-deficient animals is shown in Table IX. 



TABLE 1X^8 

 CHsC^^OOH Incorporation into Pantothenic Acid-Deficient Rat Liver Slices 



Incorporation 

 Pantothenic CoA in per gram Liver 



Group Age, weeks acid liver, units/g. of fatty acids, % cholesterol, % 



1 6 + 



10 + 



11 + 



