Chapter V 

 The Morphological Approach 



F. B. Bang 



Johns Hopkins School of Hygiene and Public Health, Baltimore, Md. 



I. Introduction 64 



II. Normal Cell 65 



A. Pinocytosis 67 



B. Mitochondria 67 



C. Nucleus 67 



D. Secretion 67 



E. Osmotic Changes 68 



III. Pathological Aspects of Cell Morphology 68 



A. Cell Surfaces 69 



B. Osmotic Imbalance 69 



C. Mitochondria 70 



D. Paranuclear Hypertrophy 70 



E. Hypertrophied orGiant Cells 70 



F. Amino Acid Deficiencies 71 



G. Cytoplasmic and Nuclear "Degenerative" Changes 71 



IV. Bacterial and Rickettsial Infections 72 



V. Psittacosis Group Viruses (Miyagawanella) 72 



A. Tissue Culture Stages 72 



B. Elementary Bodies 73 



C. Fluorescent Antibody Staining 75 



VI. Poxvirus 75 



A. Vaccinia 75 



1 . Identification 76 



2. Electron Microscopy 77 



3. Fluorescent Antibody Staining 78 



B. Fowlpox 79 



1 . Inclusion 79 



2. Effect on Nucleolus 79 



3. Development Sequence 79 



C. Shope Fibroma 80 



VII. Herpesvirus 80 



A. Intranuclear Inclusion 80 



B. Histochemistry 81 



C. Electron Microscopy 81 



D. Herpes B 81 



E. Fluorescent Antibody Staining ; 82 



VIII. Myxoviruses 83 



A. Introduction 83 



B. Filament Formation 85 



C. Living Cells 86 



D. Fixed Tissues 86 



E. Electron Microscopy 87 



63 



