Chapter VI 



Biological Aspects of Intracellular Stages of Virus Growth 



Alick Isaacs 

 National Institute for Medical Research, London, England 



I. Introduction Ill 



II. The Eclipse Phase 112 



A. The Amount of Infective Virus Recoverable during the Lag Period 113 



1. Bacteriophages 113 



2. Small Animal Viruses 114 



3. Medium-Sized Viruses 115 



4. Larger Viruses 116 



B. Attempts to Demonstrate Virus during the Lag Period by Its Antigenic 

 Properties 120 



C. The Significance of Virus Recoverable during the Lag Period 122 



III. Development within Infected Cells of Antigens Associated with Virus Multi- 



plication 126 



A. The 30 S Complement-Fixing (Soluble) Antigen of Influenza and Related 

 Viruses 126 



B. The Hemagglutinin of Myxoviruses 129 



C. Cell-Associated Antigens of Other Viruses 132 



IV. Dynamics of the Development of Lifective Virus 133 



A. Poliomyelitis Virus 133 



B. Western Equine Encephalitis Virus 134 



C. Influenza Virus 135 



D. Newcastle Disease Virus of Fowls 136 



E Herpes Simplex Virus 137 



F. Vaccinia Virus 137 



V. The Release of Virus from Infected Cells 138 



A. Western Equine Encephalitis and Poliomyelitis Viruses 138 



B. Myxoviruses 139 



C. Herpes Simplex and Vaccinia Viruses 141 



D. Mechanism of Release of Virus and Virus Filaments 142 



VI. Incomplete Virus 143 



A. Properties of Incomplete Virus 144 



B. Production of Incomplete Virus 146 



C. Partial Cycle of Virus Multiplication Produced by Incomplete Virus 149 



D. Incomplete Viruses Other Than Influenza 150 



VII. Conclusions 151 



References 153 



T. Introduction 



The information which we have at the moment of how animal viruses 

 multiply within cells is insufficient to allow us to build up a connected picture 



ill 



