Chapter VIII 

 Inhibition of Multiplication 



Frank L. Horsfall, Jr. 



The Rockefeller Institute for Medical Research New York, New York 



I. Introduction 195 



II. Prevention of Virus Infection 198 



A. Alteration of Extracellular Environment 198 



B. Inactivation of Extracellular Virus 199 



C. Alteration of Host Cells 201 



III. Inhibition of Intracellular Multiplication 201 



A. Inhibition during Latent Period 202 



1. Klebsiella Pneumoniae, Type B, Capsular Polysaccharide (Fr. B.) 203 



2. 2, 5-Dimethylbenzimidazole (MB) 205 



3. DL-Methoxinine 205 



4. 5, 6-Dichloro-l-j8-D-ribofuranosylbenziinidazole (DRB) 206 



5. ^-Fluorophenylalanine (FPA) 209 



6. Levo-y-(o-chlorobenzyl)-8-oxo-y-phenyl Caproic Acid 210 



7. 5, 6-Dichloro-l-a-D-arabinopyranosylbenzimidazole (DAB) 210 



B. Resume of Inhibition during Latent Period 211 



C. Inhibition after Latent Period 211 



IV. Inhibition of Release 213 



V. Chemical Structure and Inhibitory Activity 214 



A. Benzimidazoles and Derivatives 214 



1. Selectivity of Benzimidazoles 2] 7 



B. Amino Acids and Derivatives 217 



C. Purine and Pyrimidine Analogs 218 



D. Compounds Affecting Oxidative Metabolism 218 



VI. Modification of Cell Damage 219 



VII. Current Status of Inhibition of Multiplication 220 



References 222 



I. Introduction 



Contrary to common opinion, it is not difficult to inhibit the multiplication 

 of some animal viruses. The dynamics of the multiplication process and the 

 yield of new virus particles are affected by a wide variety of alterations in the 

 environment and metabolism of the infected host cell. They also are affected 

 by a diverse array of chemical substances that are more or less active as 

 inhibitors of multiplication. 



The genotype and age of the host, its nutritive state, the supply of vitamins 

 and other food factors, environmental conditions, infection with bacteria or 



195 



