CYTOPLASMIC INCLUSIONS 



123 



chondria or are the result of the segregation by the mitochondria of 

 materials synthesized at other points. Wermel (1925) found that 

 certain mitochondria of Actinosphaerium have a high lipoid content, 

 as shown by Ciaccio's lipoid methods, i.e., a so-called liposome rather 

 than an ordinary type of mitochondria, and concludes that they secrete 

 the lipoid reserves. Except for the fact that these granules stain weakly 



Figure 30. The association of mitochondria with the gastriole in Amoeba proteus. 

 (From Mast and Doyle, 1935b.) A, 2-6 hours; B, 6-8 hours; C, 8-16 hours; D and E, 

 16-30 hours; b and bi, mitochondria; f, fat; s, starch; v, vacuole refractive bodies; c, 

 crystals; p, pellicle of Chilomonas. 



with Janus green, they are like the intermediate lipoid bodies and could 

 perhaps be classed more conveniently with them. Zinger (1928) 

 counted the "spherical inclusions" and the mitochondria in Ophryoglena 

 and found that they are roughly proportional in number. He concluded 

 that the spherical inclusions are derived from mitochondria. Since many 

 granules of entirely different origin will increase in number under 

 favorable conditions, this conclusion cannot be considered as proved. 

 The digestive function of mitochondria rests upon two types of 

 evidence: the actual demonstration of enzymes in these granules (see 

 p. 125), and the correlation between the periodic aggregation of mito- 

 chondria around the gastriole and the type of digestion taking place 

 within. Both types of evidence are available for A. proteus, so that the 

 results of observations may be checked against a direct knowledge of the 



