CYTOPLASMIC INCLUSIONS 



141 



bodies as being identical with the neutral red bodies because of simi- 

 larity in form and distribution, and this has been widely accepted in 

 spite of objections presented by Tuzet (1931) and Subramaniam and 

 Ganapati (1938). The question was not settled conclusively until 

 Daniels (1938) applied the centrifuge to these species. She found 

 that Golgi bodies always moved to the centripetal pole of the cell, but 



39 





# 



■>ij:: 



40 



. o 



41 



43 



Figures 39-45. Dictyosomes: Figure 39, Dictyosomes from Haptophrya tnichiganensis, 

 Champy-osmic (after Bush, 1934) ; Figure 40, diagram of a dividing dictyosome of 

 Lecudina brasili (after Subramanian and Ganapati, 1938) ; Figure 41, stages in the 

 secretion of neutral fat in Ichthyophthirius multifiliis, Lorrain Smith Nile blue-sulphate 

 method, black represents blue stain, stippling represents pink (after MacLennan, 1934) ; 

 Figures 42-45, dictyosomes during the life cycle of Lecudina brasili; Figures 42 and 43, 

 intracellular stages; Figure 44, growing trophozoite; Figure 45, "association" stage, 

 either daFano or Nassonow impregnations (after Subramanian and Ganapati, 1938). 



that the bodies stainable with neutral red were never displaced. Thus 

 in this case the Golgi bodies and the neutral red granules are not 

 identical. This does not mean that no osmiophilic granules segregate 

 neutral red, since, for example, the digestive granules react to both 

 impregnation and vital staining, but it does mean that the ability to 

 segregate neutral red is not a characteristic of all the Golgi bodies of 

 the Protozoa. 



