142 



CYTOPLASMIC INCLUSIONS 



The theory that the Golgi apparatus is a universal organoid of the 

 cell, as constant in its characteristics as is the nucleus, has given rise 

 to a series of criteria requiring permanence during the whole cycle of 

 the cell, as well as similarity in form and intracellular distribution. The 

 presence of Golgi bodies in all stages of the life cycle has been demon- 

 strated in Sporozoa (Joyet-Lavergne, 1926a) as well as their origin 

 by the division of preexisting Golgi bodies (Subramaniam and Gana- 

 pati, 1938, Figs. 42-45). However, this is not universal, since neither 



Figures 46-47. The effect of centrifuging upon the distribution of cytophismic 

 granules. Figure 46, diagram of a centrifuged gregarine, F, fat, GAa granular Golgi 

 material, GAb larger Goli elements, N nucleus, K karyosome, M mitochondria, P 

 paraglycogen, C "chromidia," neutral red bodies not shown; the paraglycogen mass 

 marks the centrifugal pole. (From Daniels, 1938.) Figure 47, contractile vacuole of 

 a centrifuged amoeba, v vacuole, b mitochondria ; the mitochondria are thrown to the 

 centrifugal surface of the vacuole. (From Mast and Doyle, 1935b.) 



of the types of Golgi bodies in Ichthyophthirius ( MacLennan, 1936) 

 nor Amoeba (Mast and Doyle, 1935a) are self-perpetuating or even 

 present in all stages of the cycle, but arise de novo. Thus the Golgi 

 bodies are not universally self-perpetuating and permanent. 



The criterion of similarity in form has received considerable support, 

 but the evidence as to what this form is has been discordant. Nassonov 

 (1924) described a net-like structure around contractile vacuoles and 

 homologized this with the Golgi net. Hirschler (1927) finds that the 

 typical Golgi bodies have an osmiophil cortex and an osmiophobe 

 center, this duplex structure being called a dictyosome. The complex 

 nets around the contractile vacuoles are, according to Hirschler, aggre- 



