158 



CYTOPLASMIC INCLUSIONS 



The presence of more than one type of material in the same granule 

 has been demonstrated in several cases, in spite of the relative crudity 

 of the cytochemical methods for the demonstration of carbohydrates. The 

 oval carbohydrate bodies of Difjlugia are not completely dissolved in 

 ptyalin, and their staining reactions suggest the presence of a glyco- 

 proteid (Rumjantzew, 1922). Two types of carbohydrate reserves have 

 been reported from Sporozoa by Dobell (1925). Chakravarty (1936) 



Figure 65. The associa- 

 tion between glycogen and 

 the parabasal body in 

 Cryptobia helicis, winter 

 forms stained with iodine. 

 (After Duboscq and 

 Grasse, 1933.) 



also differentiated two sets of granules by differences in the speed of 

 destaining after treatment in iodine. These authors refer to one set as 

 glycogen, the other as paraglycogen. Two types of carbohydrate have 

 been found in Actinosphaerium (Rumjantzew and Wermel, 1925), and 

 were identified as glycogen and a glycoprotein on the basis of their re- 

 action to Best's and Fischer's stains. Peloniyxa loses its paraglycogen dur- 

 ing prolonged starvation, but since the remnants of these granules may 

 be stained with haematoxylin (Leiner, 1924), it is probable that the 

 carbohydrate is here associated with a protein. The carbohydrate bodies 



