170 CYTOPLASMIC INCLUSIONS 



and other mitochondrial stains, in each are granules which segregate 

 neutral red, and in each, with the exception of Flabelhda, are granules 

 which may be specifically impregnated by the Golgi methods. If we do 

 not press the comparison any further, we can say that chondriome and 

 vacuome are universal cell constituents but, even with the very crude 

 definition based on impregnation alone, Golgi bodies are lacking in one 

 of the five Protozoa discussed. However, this apparent uniformity 

 is reduced if these granules are compared with respect to other character- 

 istics than the so-called specific staining methods, which are in reality 

 quite crude in spite of brilliant contrasts. With respect to mitochondria, 

 the bodies range from one extreme of temporary induced granules in 

 Flabellula to two separate types in some of the Sporozoa. The neutral 

 red bodies are even less comparable: in Ichthyophthirius they are lipoid- 

 containing bodies, which are found only in association with gastrioles. 

 In Opalina they range from watery vacuoles to dense bodies of proteins 

 and are obviously not connected with any gastrioles. In Flabellula they 

 are vacuoles in which is also dissolved the material stainable with Janus 

 green. In Amoeba there are two types of neutral red bodies which are 

 of cytoplasmic origin, and both contain large amounts of lipoids and at 

 some stages proteins as well. One of these types, the refractive bodies, 

 are apparently built up from material derived from the blebs and the 

 crystals. In a structural sense also the refractive bodies are unique and 

 are much more complex than any of the other neutral red bodies. In 

 the Sporozoa, lipoid dictyosomes are weakly stainable with neutral red, 

 but in addition there are non-lipoid bodies which stain much more 

 specifically with neutral red. With respect to Golgi bodies, even if we 

 ignore Flabellula, harmony is not achieved. In Amoeba the two types 

 of neutral red bodies, as well as the mitochondria, respond to impregna- 

 tion and bleach with difficulty. A comparison between these lipoid bodies 

 and the endoplasmic Golgi bodies (intermediate lipoid bodies) of 

 Ichthyophthirius, Opalina, and the dictyosomes of Sporozoa seems quite 

 logical, until we consider that in these latter three species, these fatty 

 acid bodies are an intermediate step in the formation of neutral fat, 

 while the refractive bodies of Amoeba are finished bodies, the granules 

 of neutral fat being morphologically independent of them. None of these 

 lipoid bodies is comparable with the non-lipoidal ectoplasmic Golgi 



