PROTOZOA AND OTHER ANIMALS 909 



(1936) found that Tritrichomonas augusta sometimes survived in salt 

 solution, on slides ringed with vasehne, for nearly a year. Cleveland 

 (1928b) was able to cultivate indefinitely T. jecalis in water with feces 

 or tissue, in hay infusion, and in other ways, at temperatures from — 3° 

 C. to 37° C; and this, although it was supposed to have been derived 

 from a warm-blooded host, man. Cleveland also maintained T. augusta 

 in tap water with feces. He did not report on the ability of T. batra- 

 chorum, which Wenrich (1935) stated is morphologically indistinguish- 

 able from T. jecalis, to grow under the conditions supplied. It would 

 not be surprising if flagellates that have such marked ability to survive 

 and even to multiply outside of the host, under such simple conditions, 

 might find natural circumstances occasionally favorable to outside main- 

 tenance of life. They might, at times, be found by collectors. This does 

 not apply immediately to the studies of the species P. keilini, however, 

 as no endozoic flagellate just like it is now known, and Bishop (1936) 

 found that it would not live in tadpoles; but it raises a general question. 

 Hollande (1939) described as a free-living trichomonad the new 

 genus and species Coelotrichomastix convexas. The flagellate was found 

 in liquid manure. It has four flagella, one of them trailing and said to 

 border an undulating membrane in a deep groove of the body; but 

 there is no costa. There is a unique axostyle, ribbon-like in its posterior 

 part and located superficially near the groove, anteriorly expanded to a 

 hemispherical cupule covering a considerable part of the large nucleus. 

 All parts of the axostyle are covered by small siderophile granules. A 

 very small bacilliform parabasal body was reported. In considering the 

 characteristics of Coelotrichomastix, Hollande failed to comment on the 

 striking similarity in many respects that exists between it and certain 

 flagellates that have been assigned to the genus Tetramitus. This cannot 

 fail to impress the reader of the accounts by Klebs (1893), Bunting 

 (1926), Bunting and Wenrich (1929), and Kirby (1932a). In those 

 papers, furthermore, especially the second and third, one will find 

 facts that suggest the possibility of a diff^erent interpretation of certain 

 unexpected characteristics described by Hollande. In assigning "Tricho- 

 mastix" salina, originally described by Entz (1904), to Coelotricho- 

 mastix, Hollande made no comment on the writer's account of what 

 seemed to be the same flagellate under the name Tetramitus salinus 

 (Entz). 



