TUNICATA OF THE L.M.B.C. DISTRICT. i bs 
Botryllus schlosseri, Pallas. 
On September 8th, 1888, I found on the South Spit at 
Puffin Island several very large colonies of this. species, 
one measuring 12 cm. by 8 cm., and nearly covered with 
small colonies of Polyzoa, both encrusting and branched, 
and of Hydroids, and with many Amphipoda (Triteta 
gibbosa) and Lemodipoda adhering. It has also been 
found at Puffin Island in February, 1888, and at Black 
Point, Anglesey, in October, 1887. 
There are various specimens of Botryllus from:—Douglas 
Bay, dredged, August, 1886; Puffin Island, shore, June 
26th, 1887; and off Port Erin, ‘“‘ Hyzna”’ expedition, 1888; 
which were not sufficiently examined when alive, and 
cannot now be identified with certainty. 
Botryllus smaragdus, Milne-Edwards. 
Found on the Beacon Rocks, Puffin Island, on June 
26th, 1887, and July 24th, 1888. These colonies are 
very fine, and vary a good deal in their colouring. The 
pigmented corpuscles in the blood are of various tints of 
sreen, blue, yellow, red, and opaque white. The larger 
lateral tentacles of the ascidiozooids (described and figured 
in the last report) are occasionally found to be branched 
at their tips. In several cases I have found an Acineta 
adhering to the tentacles at the base of the branchial 
siphon; and a small Hydrachnid, coloured dark blue and 
green like the colony, is found running over the surface. 
Botrylloides rubrum, Milne-Edwards. 
This species 1s not uncommon at low tide on the shore 
at Puffin Island. It is also found, I believe, on the shore 
at Llandudno. We have brought it up on a long fishing 
line set off the rocks below the Biological Station. 
Botrylloides leachti, Savigny. 
A large colony was collected on the South Spit, Puffin 
10—2 
