Ill 



XENO-INDUCTIONS 



435 



TABLE 6 



MAIN INDUCTIVE ACTIVITY OF SUCCESSIVE FRACTIONS OF GUINEA PIG KIDNEY 



Compiled from Yamada and Takata, 1955 



Fractionation procedure 



(— > = centrifugation, 



I = precipitation) 



RJVA-P mg Acren- General Chorden- 

 pro mg N cephalon notogenesis cephalon 



Remarks 



1 . Blocks of tissue + ethanol 



(a) preserved 2 h. to 5 days 



(b) preserved 17 h. to 45 days ? 



2. (a) Homogenate 



in 0.14 M NaCl super- 

 natant + ethanol 18.2 

 (Preserved i-io days, 

 and washed) 

 (b) idem + heat 



3. Id. preserved 37 days at 5° C. id.? 



4. Supernatant of 2a, [ pH 4.2, 

 -^, reextracted, — » 

 redissolved NaCl, | ethanol. 



washed 



31-7 



5. Supernatant of 2a, | pH 4.2, ±31.2 



reextracted, — > redissolved 



i 3 A/ NaCl (36 h.) 

 [ ethanol 



6. precipitate of 5 resuspended ca. 10.6 



in 3A/ NaCl, [ ethanol (variable) 



7. suspension of 6 filtered, 



pH 4.2 + ethanol 13.9 



8. suspension of 5, [ pH 4.2 



+ ethanol ? 



9. Supernatant of 2a, J, pH 4.2, 



-^, supernatant [ by 



ethanol 13.1 



ID. (2b) supernatant [ by heat (like 2?) 



— + 



+ + + 



+ + + 



+ 



+ + + 

 + + + 



+ 



+ 



+ 

 + 



+ 



with 45 days 

 acren. + + + 



+ 



optic vesicle 

 atypical 



The repetition of the sign + has only a meaning inside the horizontal line concerning 

 one single specimen ; it is intended to show the numerical predominance of one type of 

 structure over another. 



Literature p. 



