ES, 
their vitality in water much longer than do those organisms 
encountered in this country. Our experiments were not 
made with laboratory cultures, but with mixtures of feces 
and water which contained all the usual varieties of intes- 
tinal organisms, as well as streptococci. Consequently, these 
two results are not strictly comparable. 
A second series of experiments was made on a much 
larger scale. In the compound of the laboratory there is 
a very large tank or pond which has a surface of about an 
acre and a half. This tank was carefully polluted with a mix- 
ture of cowdung, feces and effluent made up in large quanti- 
ties. This evil mixture was spread over the surface of the 
tank just at sunset. The mixture always contained strep- 
tococci in large numbers. Morning and evening of the sub- 
sequent day samples were taken from the surface and from the 
bottom of the tank. The results are given below in tabular 
form :— 
TABLE V(a). 
EXPERIMENT No. I—Tank Polluted, 15-12-10. 
Surface sample I c.c. 10 A.M., 16th, a few chains. 
AEM TOU 42 ~ 2: * 
:. 3 »  IOA.M., 17th, no streptococci. 
Bottom samples Ic. morning and evening, 16th, present. 
Lec. TO A.M.,.1 7th, present. 
4 P.M., 17th, absent. 
a»? a7 be) 
” »” 
” a»? 
EXPERIMENT No. I]—Tank Polluted, 3-4-11, 4 P.M. 
Surface sample I c.c. 8 A.M., 4th, present. 
Bottom 49 s . » ‘present, 
Surface os » 4P.M., 4th, absent. 
Bottom s a 5 =) Present 
Surface 7 »  98A.M., 5th, absent. 
Bottom PP A ei 3° present, 
Bottom _e,, ,»  8A.M., 6th, absent and all subsequent analyses. 
EXPERIMENT No. I]]—Tank Polluted, 1-5-11, 4 P.M. 
Surface sample I c.c. 8 A.M., 2-5-11, absent. 
Bottom . A ie 3 present, 
Surface 5 | AB: ,, absent. 
Bottom Zs i y i; “present. 
