79 
evening of the 5tha further change had come over the organ- 
isms in the emulsion, bacillus lactis erogenes largely pre- 
ponderated, acidi lactici, Grunthal and cloace began to appear, 
and another organism No. 67 was present for the first time. 
A sample taken on the evening of the 7th shows still further 
alterations. A new organism, namely, No. 33 which, it should 
be noticed, belongs to MacConkey’s group No. 2, and possesses 
reactions almost identical with those of coli communis 
itself, appeared for the first time. Unfortunately for three 
days, only the daily investigation as to the presence of fecal 
organisms in 5 c.c. was taken; on two more occasions the 
operation of putting the organisms through the sugars was 
carried out. But these results show that organism No. Io1 
preponderated and outlived most of those above-mentioned. 
These results, incomplete as they are, give a fair idea of the 
order in which the organisms died off under the action of the 
sunlight. The important point to notice is, that in a feces 
crowded with coli communis, these organisms had entirely 
disappeared in less than eighteen hours, so that the various 
other more resistant types were easily isolated. 
Experiment No, 2. 
Calf Dung—7-3-1910. 
Taken at 11-45 A.M., before exposure (Lactose fer- Vesiculosus 
menters in ‘OOOI cc.) ae ees 
Vesiculosus 
'" €L. zrogenes 
j Vesiculosus 
“" UL. erogenes 
Vesiculosus 
L. erogenes 
le 
Mutabilis 
Vesiculosus 
12-45. 55 na 2° .. 4, Coscoroba 
eras 
(eee 
” I 3 .- 2° .. + L. erogenes 
Gasiformans 
) L. erogenes 
" '(Gasiformans 
a5 I2 noon 
~ 12-15 P.M. 
HO FO WH 
on 
to 
OH 
oS | 
NwaABA BR W 
