72 
Experiment No. 3.—Contd. 
- Vesiculosus of 
Taken at 11-30 A.M., On 18-3-1910 wis ; ahs 
mat 11-30 A.M., On 18-3-19 ) Granth 3 
Le eit Vesiculosus .. 6 
7 “* | Coscoroba en 4 
* I2-40, 2 P.M., 2-30 P.M., 5 P.M. .2 sterile in Joe. 
Note.—The very rich mixture at the commencement of the experiment 
and the gradual weeding out of the varieties till Vesiculosus and Coscoroba 
remained at the end. 
Experiment No. 4—Cowdung. 
Emulsion before exposure contained .. 1 coli communis, I cloace, 4 cos- 
coroba, 4 Grunthal. 
After 1 hour’s exposure showed -- 3 Coli Cofimunis, 3 Grunthalie 
coscoroba. 
= = s2 OES: Bet os .. 8 Grunthal, 1 coscoroba, 1 cloace. 
ae - ” -.  6Grunthal, 2 coscoroba, 2 No. 100. 
oe) Bf es c i .. 2 Grunthal, 8 coscoroba. 
At tI o'clock on the 2nd day (after 
about 10 hours’ exposure) 24 1O%arunthal: 
At's5on the'second day'\~_.. sh = BO: ide: 
Subsequent samples contained no organisms 1n 20 cc. 
Note.—Coli communis survived one hour, but not 2 hours’ exposure to 
sun ; late appearance of bacillus cloace ; final bacillus that remained was 
bacillus Grunthal ; even this disappeared after about 3 days’ exposure. 
In the following experiments a single specimen of cow- 
dung was taken, but a layer of sterile sand was placed at the 
bottom of the dish :— 
Experiment No. 5. 
Emulsion before exposure contained .. 5 lactis aerogenes, 2 No. 100, 3 No. 67. 
After 1 hour’s exposure showed .. § lactis erogenes, 3 No. 67, 2 No. 100. 
i 2 hours’ + et .. 7 lactis erogenes, 2 No.100, 1 Grunthal. 
P 3 - a ae .. 10 lactis ezrogenes. 
aes - 4s 7 .. 3 lactis erogenes, 3 No. 67, 2 cloace, 
1 Grunthal. 
Noon of the second day (after 10 3 lactis erozenes, 4 Cloace, 1 neapoli- 
hours’ exposure) showed tanus, 2 coscoroba. 
Evening of second day showed .. 5 lactis erogenes, 3 cloace, I coscoroba, 
I neapolitanus. 
Evening of third day and subsequent samples were sterile in 20 cc. 
Note.—This experiment is interesting on account of the preponderance 
of lactis wrogenes from start to finish, showing that these organisms are to 
be looked upon as one of the fairly resistant ones. Note also the gradual 
