73 
increase of bacillus cloace as the experiment proceeds. Bacilli Nos. 100 
and 67 were present at the beginning and persisted for a considerable length 
of time ; unfortunately the experiment was spoilt by our not taking sufficient 
samples on the third day to observe the last surviving organisms. 
Experiment No. 6—Cowdung. 
In order to provide a larger number of varieties a mixture 
of 10 samples of cowdung were taken and the emulsion was 
exposed in the ordinary way :— 
Emulsion before exposure contained .. I coli communis, 2 coscoroba, 4 
Grunthal, 1 cloace, I acidi 
lactici. 
After 1 hour’s exposure showed - 5 Grunthal, 1 neapolitanus, 3 
No. 101, No. 6. 
2 Dours’ a at ie 4 Grunthal, 1 lactis «wrogenes, 2 
cloace, I neapolitanus, I cos- 
coroba, 1 No. 67. 
en 3 m ss a me 3 Grunthal, I coscoroba, 2 lactis 
zrogenes, 2 No. 67, 1 No. Ior, 
t No. 6. 
Noon on the second day showed a 5 cloace, 3 lactis «rogenes, 2 
No. Io1. 
At 3 x ee iiap ORO oe 
At 5 a * se te cleace: 
Noon of the third day and subsequent samples were sterile in 20 cc. 
Note.—Coli communis did not survive after one hour’s exposure ; bacil- 
lus grunthal did not in this mixture persist till the end, but appears to have 
disappeared sometime prior to noon of the second day ; bacillus No. 67 and 
bacillus cloace both appeared to survive till quite late ; the rare organism 
No. 6 was present in this mixture and survived after three hours’ exposure 
to sun. 
Experiment No. 7. 
A single sample of human feces with a layer of sand 
placed at the bottom of the dish :— 
Emulsion before exposure contained .. 3 lactis erogenes, 2 Grunthal, I 
No. 67, 4 acidi lactic. 
After 1 hour’s exposure showed a 1 colicommunis, 2 acidi lactici, I 
No. 67, 6 Grunthal. 
i» 12 Botre? = i is 1 lactis wrogenes, 1 acidi lactici, 
1 cloace, 1 coscoroba, 6 Grun- 
thal. 
