74 
Experiment No. 7—Contd. 
Noon of the second day (after 8 hours’ 
exposure) showed = me I lactis erogenes, I coli communis, 
2 acidi lactici, 6 Grunthal. 
Evening of the second day showed Ae 1 acidi lactici, I neapolitanus, 3 
coscoroba, 5 Grunthal. 
Noon of the third day showed -. 7 coscoroba, I cloace, 2 Grunthal. 
Evening of the third day showed .. 3 acidi lactici, § coscoroba, 2 Grun- 
thal. 
ys fourth Ms a 3 acidi lactici, 3 coscoroba, 3 Grun- 
thal, 1 cloace. 
¥ fifth x, om 3 Grunthal, 5 coscoroba, I acidi 
lactici, I pneumonie. 
mn sixth < Las 6 Grunthal, 1 cloace, 3 coscoroba. 
Be seventh “a ots 7 Grunthal, 2 coscoroba, I neapo- 
litanus. 
Subsequent samples proved sterile in 20 cc. 
Note.—Disappearance of organisms less rapid than usual due to the 
presence of sand ; much later disappearance of coli communis, namely, at 8 
hours ; no subsequent appearance after this. This delayed disappearance 
is due to the presence of sand in the bottom of the dish ; the usual appearance 
and disappearance of cloace, lactis erogenes, and particularly coscoroba, 
which at one time decidedly predominated ; increase in number of Grunthal 
tor the first 4 or 5 analyses, then slight decrease followed by final survival 
of these organisms ; coscoroba and neapolitanus in small numbers also 
appeared towards the end. 
Experiment No. 8. 
Mixture of 5 samples of human feces with a layer of sand 
at the bottom :— 
Emulsion before exposure contained .. 1 colicommunis, 3 acidi lactici, I 
cloace, 5 Grunthal. 
After 14 hours’ exposure showed ce tcoli communis, 5 Grunthal, 3 
acidi lactici, 1 cloace. 
I acidilactici, 2 No. 67, 7 Grunthal, 
21 
aes ” ” ” 
1 
4 Z FA 2 acidilactici, 1 cloace, 7 Grunthal. 
Noon of the third day sn ss 2 coli communis, 3 acidi lactici, I 
coscoroba, 4 Grunthal. 
I-vening of the third day showed .. 9Grunthal, 1 cloace. 
fourth bg a 8 Grunthal, 2 cloace. 
fifth ce zs 5 acidi lactici, 5 Grunthal, 
“ sixth ms 3 acidi lactici, 7 Grunthal. 
seventh 7 is Sterile. 
Note.—Very slight variation in number of Grunthal from beginning to 
end of the experiment, the persistence of coli till the end of the second day, 
