75 
but not longer (as in other experiments); comparatively late persistence 
of acidi lactici ; usual appearance and disappearance of No. 67 and _ cloace. 
Note the alteration in numerical relation of bacilli to one another in the 
first 14 hours is n7i. 
Experiment No. 9. 
A single sample of human feces with a layer of sterile 
sand at the bottom of the dish :— 
Emulsion before exposure contained .. 3 acidi lactici, 2 coscoroba, 4 Grun- 
thal, 1 No. 6. 
After 1 hour’s exposure showed be 1 lactis wrogenes, 2 coscoroba, 
1 No. 75, 6 Grunthal. 
2 hours’ . irs tes 3 lactis erogenes, 8 Grunthal, 2 
No. 75; 
2lactis wrogenes, 2 cloace, I 
No. 75, 2 coscoroba, 3 Grunthal. 
7 Grunthal, 1 coscoroba, 1 No. 67, 
Ww 
. 
~ 
? 
tant 
” ” ” ” 
I No. 6. 
Noon of the second day (after 11 hours’ 
expostire) .. es ry 7 Grunthal, 2 coscoroba, I lactis 
erogenes, 
Evening of the second day .» +6 Gninthal,, - 3. acidilactici, 3 
No. 161, 
Noon of the third day ae = 5 acidi lactici, 5 Grunthal. 
Evening of the fourth day uA 4 acidi lactici, 1 cloaez, 5 Grunthal. 
= HAE 5) . “fis 5c 3 acidi lactici, 7 Grunthal. 
a sixth ., 8 Grunthal, 2 No, 101. 
a seventh, .. we No organisms. 
Note.—Persistence of acidilactici from start till quite late ; appearance 
and disappearance of lactici wrogenes; appearance and disappearance otf 
organism No. 75 ; presence of rare organism No. 6 and its persistence for five 
hours at least; final survival of bacillus Grunthal. 
Besides exposing to the sunlight emulsions of faces a few 
experiments were done on some special samples of water only. 
Experiment No. Lo. 
The river Adyar flows close to the King Institute. It 
is very shallow. It is considerably polluted all along the 
banks and very highly polluted just below the bridge where 
many hundreds of washermen wash the clothes of the inhabit- 
ants of the city :— 
