78 
One hour afterwards the analysis reveals an entirely different 
state of affairs. Only 2 Grunthal persist, 4 coli communis 
and sundry other organisms have made their appearance. 
If a large number of Grunthal had not disappeared during 
the first hour, they would have still predominated in the 
second, third and fourth analyses to the exclusion of other 
varieties. Consequently it is certain that quite a fair per- 
centage of this bacillus Grunthal disappeared within the first 
hour. But as the experiment proceeds the usual state of 
affairs declares itself, namely, cloace begins slowly to pre- 
dominate, and continues to do so up to within the last two 
analyses, when bacillus Grunthal again comes to the front, 
and in the final analysis not a single other organism could 
be isolated. It should be observed that this emulsion was 
made from 5 different specimens of human feces, and a 
layer of sand was placed at the bottom of the dish. Thus 
we are absolutely forced to the conclusion, that, although a 
large number of the organisms, included in the term bacillus 
Grunthal, were sufficiently susceptible to die off in one hour’s 
exposure, the remainder were very resistant. Now it may 
be argued, that considering bacillus Grunthal was by far the 
most numerous organism present at the beginning of the 
experiment, the following explanation would completely 
satisfy all the facts observed. Let it_be granted that in a 
large number of individual bacilli of the same species resist- - 
ant and delicate specimens exist, then any organism that 
preponderates at the beginning of the experiment will survive 
to the end, because the more organisms there are at the com- 
mencement, the greater is the chance of resistant ones being 
included amongst the number of those experimented on. 
It must be admitted that such explanation would account 
for all the results in Experiment 11. But if this rule be ap- 
plied universally, the results obtained would not be as uniform 
as they are. Further, other experiments disprove this theory. 
Thus, in the case of bacillus Grunthal; other experiments 
could be given showing the steady increase in the number 
from beginning to end. In Experiment No. 12, note not a 
single Grunthal. was isolated in the first analysis ; yet it sur- 
vived all others. | 
