79 
Experiment No. 12—Cowdune. 
5 
Emulsion before exposure contained .. 4coscoroba, 1 cloace, 1 coli 
communis, 3 neapolitanus, 1 
acidi lactici. 
After 1 hour’s exposure showed me 7 coscoroba, I Grunthal, 1 cloacex, 
: I neapolitanus. 
=) 4hours’ if Pe a 7 lactis zrogenes, 3 Grunthal. 
Noon of second day 7: -« 40 Grunthal 
Evening of second day oe ia, BO do. 
Noon of third day a ve tO do. 
Evening of third day et er LO do. 
Noon of fourth day oe ori KO do. 
Evening of fourth day os ee > do. 
In subsequent 20 cc. samples there were no organisms. 
Note.—Disappearance of coli communis after 1 hour’s exposure ; fairly 
rapid disappearance of neapolitanus ; these organisnis did not survive longer 
than a few hours ; appearance of bacillus Grunthal after one hour though it 
was not detected in the sample before exposure ; appearance of lactis erogenes 
sometime before 4 hours’ exposure, but its disappearance before noon of 
second day ; the longer persistence of bacillus Grunthal as compared with 
other organisms. 
Experiment No. 13—Cowdung. 
Emulsion before exposure contained .. 3 coscoroba, 2 coli communis, 2 
acidi lactici, 1 Grunthal. 
After 1 hour’s exposure showed = 1 coli communis, 7 coscoroba, 2 
Grunthal. 
» 2 hours’ =f ot xe 3 coscoroba, 6 Grunthal, 1 cloace. 
re ee ie 2 .. 10 Grunthal. 
The emulsion became free from lactose fermenters by noon of the 
second day. 
Note.—That bacillis coli communis survived for one hour, but not for two; 
that bacillus Grunthal was the last survivor ; the late appearance of cloace. 
If further proof be necessary, the following points are 
quite convincing: (1) In Experiment No. 1, the preponder- 
ating organism was coli communis; it disappeared entirely 
before the second analysis was taken, and never reappeared ; 
(2) in very few of the emulsions before exposure is bacillus 
cloace shown to be present. In nearly every sample bacillus 
cloace appeared later on, persisted for a certain length of 
time, and either remained to the end or disappeared just 
before bacillus Grunthal. These two important facts are, 
