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motility by the action of the sun, and does not recover it easily 
by growth in the media enumerated above. 
There are one or two practical hints on laboratory organis- 
ation, which make the method given above a very simple 
matter, although the description would seem to indicate that 
it is most tedious and complicated. For these ‘‘ tips ’’ we are 
indebted to Dr. A. C. Houston. 
(1) It is recommended to provide oneself with a large 
number of iron wire loops, and also a similar number of short 
lengths of iron wire for inoculating various tubes from one 
colony. These iron wire loops and rods take the place of the 
usual platinum loop. They can be sterilized in the Autoclave 
in bunches of a 100 and kept in test tubes ready. A separate 
loop or rod is used for each bacteriological operation. The 
saving of time thereby effected is obvious. 
(2) Small racks are necessary for holding six test tubes for 
the six tests described. This should be made very small so 
as to take up a minimum of room in the incubator; those 
used in our laboratory contain 12 test tubes, that is two rows 
of Six. 
(3) It is very convenient to use a different coloured wool 
plug for each of the six test tubes containing the six different 
sugar media. 
(4) For such expensive sugars as dulcit and adonit, we 
use very small test tubes on the score of economy. Those 
measuring 3” by 3” are quite large enough to give the reaction 
satisfactorily and their use effects a large saving. 
(5) Always have plenty of sterile pipettes, bent rods, etc., 
ready at hand. This saves a great deal of time and temper. 
By the use of the coloured wools and by numbering the racks 
serially, all labelling or even numbering of tubes is avoided. 
