APPENDIX. 6. 
List OF MEDIA USED IN THE WORK DESCRIBED. 
I. (Stock solution A).—Bile salt glucose peptone neutral 
red broth— 
Peptone A .. 60 grms. 
Sodium taurocholate 15 __,, 
Glucose ies eC ae, an Rae 
Water - af... PE we 
Neutral red .. -. / I0c.c: of 5 ‘per cent. soliwtiaa 
Normal sodium .. |. Till neutralised as indicated 
Carbonate solution .. § by the neutral red. 
(B Solution)— 
2 parts of A solution + I part of wate:. 
(C Solution)— 
I part of A solution + I part of water. 
(D Solution)— 
I part of A solution + 2 parts of water. 
II. Bile salt lactose peptone neutral red broth— 
Same as above, lactose being substituted for glucose. 
III. Bile salt lactose peptone agar— 
Peptone ae 2. 20 grms. 
Sodium taurocholate ae 
Lactose ae aay “Ae 
Pear... ae since BOO eee 
Water ra ec See: 
Neutral red .. -- Ioc.c. of 5 per cent. solution, 
Till neutral point is reached 
Normal sodium rhe 2 
; as indicated by the neutral 
Carbonate solution .. i q 
IV. Nutrient agar— 
Agar .. a -- 20grms. 
Lemco iz es ae 
Peptone a eee Seas 
Sodium Cilorida <<. & ,, 
Water me Ae. I litre. 
