161 
Standardised to + Io using normal sodium carbon- 
ate solution. 
V. Nutrient broth— 
Peptone ne Jo V TOMS. 
Sodium, chloride. :.. 5-3, 
Lemco aie tied’ 5S cae 
Water Re ou) © litre: 
Standardised to + 10 using normal solution of so- 
dium carbonate. 
VI. Bile salt peptone neutral red sugar medium— 
Peptone e Pa ROLE TES. 
Bile salt ue eM a ies 
Sugar he ae he es eee 
Water = Sy 1 LBEE: 
Neutral red .. .. 5 6¢.c. of 5 percent. solution, 
To prepare different sugars, the particular sugar 
is substituted in the column of sugar in the above 
solution. 
The following sugar media are made— 
I. Saccharose. 3. Adonite. 
2: Duleite: 4. Inulin. 
VII. Glucose medium for Vosges and Prosk. reaction— 
Peptone e sy 20. 2rms: 
Glucose 5 Reem aly Bat te Be 
Water Zt Pye Lee: 
VIII. Strong peptone solution for vibrios— 
Peptone Ss .« LOO-2rms. 
Sodium chloride... 50% —,, 
Water ste Se MORO ees 
Io c.c. of this in go c.c. of suspected water converts the 
latter into I per cent. peptone water. 

THACKER, SPINK & Co., 6 MANGOE LANE, CALCUTTA. 
