rHE MYXOSPORIDIA, OR PSOROSPERMS OF FISHES. 293 



Plate 10. 



Figs. 1-3. Ghigea anomala. 



la-h. (After Gluge. X 1.) _ 



la, h. Showing Gastero.steus aculeatus witli tumors on sides of body and on tail. 

 X 1. 



le-e. Spores variously magnitle<l. X 255-810. 



If, g. The same "coaguhitud." 



l/i. Cyst membrane. 



2. Section showing, from above downward, subcutaneous connective tissue, cyst 

 membrane, protophismic contents of cyst, and spores (after Th(51oban. X 1). 



3a-!. Group of spores : a, h, fresh ; c-i, safranin stained ; c, d, spores with 1 

 nucleus; e, f, with 2 nuclei; g, with 3; h, i, with 4 (after Th^lohan. X 1). 

 Figs. 4-5. Thelohania contejeani (after Henneguy and Thdlohan). 



4. Longitudinal section of diseased crayfish muscle (X 1). 



5a. Spores in sporophorous vesicle, and free (X |). 



5b. Individual spore, more highly magnified (X |). 

 Fig. 6. T/fe/o/iaHia oc^os^jo»a (after Henneguy. X 1). 



6a. Sporophorous vesicle with spores. 



6b. Individual spores. 



6c. Longitudinal section of diseased muscle of Palwmon rectirosfris, showing 

 sporophorous vesicles between the separated fibrillie. 



6d. Portion of c more highly magnified. 



Plate 11. 



Figs. 1-5. Tlielohania octospora (after Henneguy and Tln^lnhan. X 1 except fig. 5). 



1. Transverse section of entire abdouien of a badly diseased Pa/awoH revtirostria, 



showing, opposite the letters, the following : vi, m, ati'ected muscles ; dt, diges- 

 tive tube; n, nerve cord; c/, sections of the claws. 



2. Longitudinal section of muscle showing the dissociation of the fibrillar. 



3. Transverse section of diseased muscle. 



4. A part of fig. 2, more highly magnified, showing fibrillaj with very clear stria- 



tion, and the sporophorous vesicles. 

 5a-d. Showing the spores: b, in the fresh state showing the vacuole; a, c, d, 

 after action of ether; a, with the filament partially, o and d with it com- 

 pletely, extruded (X f). 



Plate 12. 



Figs. 1-2. Thelohania giardi (after Henneguy and Th<^lohan). 



1. Spore formation (X S). 



la. Young pansporoblast. 



\h. Pansporoblast whose nucleus has lost its membrane and presents itself under 

 the form of an equatorial plate. 



Ic. Pansporoblast whoso nucleus has segmented into 2. 



Id. Pansporo!)last the protojjlasm of which has segmented into 2 uninucleate 

 plasma hemispheres. 



le. Pansporoblast in the iv stage; fresh state. 



If. Pansporoblast in the iv stage, the augumentation of size of nuclei and change 

 in disposition of chromatin preliminary to division. 



\g. Pansporoblast in the IV stage; nucleus in repose. 



1/i, i. Pansporoblast in the viii stage; different dispositions of the sporoblasta 

 (the 8th in i is not represented, being hidden by the others). 



Ik. Sporopliorous vesicle inclosing 8 ripe spores. 



\l. Pansporoblast iuclosiiig 4 normal spores, and 2 bodies each formed by the 

 soldering together of 2 spores by their large ends: a, thickening of the pan- 

 sporoblast membrane; h, spores soldered; 8, normal spores. 



l7H, n. 2 sporoblasts with crescentic nucleus. In the concavity of the Latter, a 

 clear vacuole. At n a small protoplasmic button i)roje(ts into the vacuole. 



lo. Spores in fresh state sliowiug at the large end a clear vacuole and at the small, 

 a brilliant point corrcspoiidiug to the capsule. 



Ip. Spores showing the vacuole and the longitudinal shell-stria<. 



Ig, r. Spores after action of sulphuric acid: q, filament incompletely unrolled; 

 r, filament completely unrolled. 



