ap) 
was grown. ‘These were selected from the standpoint of their 
ie third generation. As a 
— 
high resistance or susceptibility in t 
general rule, if the third generation progeny was resistant, the 
fourth generation descendants were also resistant. In a corre- 
sponding way, susceptibility among the third generation progenies 
was manifested in the following generation. 
During the past year the extensive results obtained with hybrids 
between Fulghum and Black Mesdag were published. There were 
four crosses and second, third, fourth and fifth generation plants 
were inoculated with the Fulghum race of loose smut. In the 
second generation 500 plants were inoculated, of which 90 (18 per 
cent.) became infected. There were two groups of third genera- 
tion progenies. One group included survivors from the inoculated 
second generation plants. When these were tested, 47 proved to 
he resistant, 89 segregating and 5 susceptible. The latter may be 
explained as due to escapes in the second generation. The second 
group of progenies descended from uninoculated second genera- 
tion plants. These were classified as 27 resistant, 47 segregating 
and 24 susceptible. The data indicate that resistance 1s dominant 
and segregation occurs on the basis of a ratio of three resistant 
to one susceptible. 
‘The most interesting results with the fourth and fifth generations 
were concerned with the resistant progenies. The resistance evi- 
dent in the second and third generations was continued throughout 
the fourth and fifth generations. 
Cultural Characteristics of the Oat Smuts 

Mr. L. Gordon Utter has continued his studies on the charac- 
teristics of both loose and covered smuts of oats when grown on 
artificial media in flasks. 
Int 
and conidia were originally isolated and cultured, successive trans- 
— 
1e culture studies undertaken, both the single chlamydospores 
fers being made to new culture media from time to time. Obser- 

vations were made on the color and topographic characteristics of 
cultures from two races of loose smut and three of the covered. 
The results are in accord with those previously reported (/reok- 
lyn Bot. Gard. Record 24: 55,56. 1935). Continued transferring 
of cultures in triplicate of the various races showed that the new 
