vil QUEENS IN CONFINEMENT 131 
caught a ¢errestvis queen that was searching for a 
nest, and confined her in a box in which I had placed 
an artificial nest, keeping her supplied with food. 
She showed several signs of preparing for a family ; 
for instance, she would sit in the nest a good deal, 
and was careful not to soil it, and also she made a 
great fuss when disturbed. But later these signs 
disappeared, and she became restless. 
I repeated the experiment with a good many 
terrestris queens : some gave the same result, others 
took no interest whatever in the nest. 
I next tried placing two ¢errestrzs queens instead 
of one in each box. With these I got much better 
results, and many of them went so far as to lay eggs. 
Although the queens were often afraid of one another 
when first put together, they soon became friendly, 
provided they were allowed to have sufficient room, 
and the eggs were usually laid about a week after 
confinement had commenced. But unfortunately, 
about the time that the eggs were laid, sometimes a 
day or two before, sometimes a day or two after, one 
of the queens always killed her companion. The 
surviving queen at first paid every attention to her 
brood, but within a week she always deserted it, 
although I took the greatest care not to disturb her, 
and supplied her with plenty of food, both honey 
and pollen. In several cases, however, it was 
ascertained not only that the larvae had hatched, 
but that they had been fed by the queen, and had 
begun to grow. 
Hoping that the queen’s affection for her brood 
