260 THE HUMBLE-BEE x 
A looked after her brood energetically for 
several days and laid more eggs, but 4 was 
generally drowsy. On May 13, however, both 
queens were incubating the brood. The paralysis 
of A’s hind left tibia had increased, and, in con- 
sequence of her inability to use this limb, the left 
side of her abdomen had become clogged with wax. 
May 14. Ata distance of a quarter of an inch 
from the brood a waxen cell has been constructed 
2 in. in diameter and tin. deep. This, I think, was 
an attempt at a honey-pot, but no honey was ever 
placed in it. 
About this date two or three batches of eggs 
were laid in cells adjoining the first egg cell. The 
queens continued to live on friendly terms with one 
another, sometimes the one sometimes the other 
incubating the brood, until May 26, when 4 
killed 4. Such a long-continued friendship of 
two incubating queens was unique in my experi- 
ence, and I attributed it to the fact that both were 
maimed. 
On May 29 4A’s paralysed tibia, which had 
been dead and useless for some days, dropped off. 
An enormous quantity of wax had now accumulated 
on the left side of her abdomen, and as this greatly 
impeded her movements, I determined after I had 
examined it to remove it. The wax had exuded 
from the base of each of the dorsal segments except 
the 1st and the 2nd, and formed lumps which 
extended nearly half-way across the abdomen. The 
lump at the base of the 5th segment was by far the 
